Journal: bioRxiv
Article Title: Comparison studies between Cesium-137 and X-ray irradiators in epithelial injury using in vitro and in vivo models
doi: 10.64898/2026.04.17.719248
Figure Lengend Snippet: RT-qPCR analysis of CDKN1A ( a, f, k ), KLF5 ( b, g, l ), KLF4 ( c, h, m ), BAX ( d, i, n ), and BCL2 ( e, j, o ) in HCT116, RKO and DLD-1 calculated as a fold change at 6, 24, 48 and 72 h after irradiation. HPRT1 was used as a housekeeping gene (control). RT-qPCR was performed as described in the “Materials and methods” section. Data points represent the average of three independent experiments, with the mean ±SD indicated. Significance was determined by the Student’s test followed by an analysis of the normal distribution (Tukey’s test), *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
Article Snippet: Commercially available TaqMan primers detecting mouse ChgA (Mm00514341-FAM), Alpi1 (Mm01285814-FAM), Olfm4 (Mm01320260-FAM), Cdkn1a (Mm00432448-FAM), Lgr5 (Mm00438890-FAM), Mki67 (Mm01278617-FAM), Actb (Mm04394036-VIC) and human CDKN1A (Hs00355782-FAM), KLF4 (Hs00358836-FAM), KLF5 (Hs0000000-FAM), BCL2 (Hs01048932-FAM), BAX (Hs00180269-FAM) and HPRT1 (Hs02800695-VIC) transcripts were used.
Techniques: Quantitative RT-PCR, Irradiation, Control